Sticker tubes: Special tubes with middle nodes and one of the soluble substances is placed in the upper part of the tube. For example; The sulfur tablet is put into the tube, melts at 120 ° C in 5-8 minutes and passes to the bottom of the tube. This indicates that the temperature has reached the desired level and sterilization is complete. Benzoic acid 120, femacetin used for this purpose melts at 135 ° C.
Biological indicator: High temperature resistant spore bacteria are used as biological indicators. For example, Bacillus subtilis, Bacillus stearothermophilus etc. like. Bacteria spores are placed in special tubes in the middle of the autoclave where heat and steam can hardly reach. Death of the bacteria with spores in 12 minutes at 121 ° C indicates that the sterilization has been done completely. After sterilization, the bacteria are cultured and planted into broth with thioglycolate or minced meat. If no bacteria grow at the end of 7 days, it indicates that sterilization has been completed.
Browne sterility control tubes: These are tubes containing color-changing liquids and placed in the middle of the autoclave. Changes color in autoclave at 115 ° C, 25 minutes, 121 ° C, 15 minutes. Turning from red to green at the end of these periods indicates complete sterilization.
T.C. Milli Eğitim Bakanlığı Sağlık Hizmetleri Sterilizasyon Ankara, 2016